Immunofluorescent Microscopic Study of Meiosis in Zebrafish

In recent years, zebrafish (Danio rerio ) has been used as a model vertebrate organism for studies of human disease, development, and genetics. This chapter describes detailed methods for the preparation of whole-mount meiotic oocytes and spermatocytes as well as cryostat sectioning of ovaries and testes for immunofluorescence microscopy studies of zebrafish.