Making cRNA for Microinjection and Expression of Fluorescently Tagged Proteins for Live-Cell Imaging
Fluorescently tagged proteins have become a crucial weapon in the armory of a su
Fluorescently tagged proteins have become a crucial weapon in the armory of a successful cell biology laboratory. This chapter describes how to produce cRNA coding for a fluorescently tagged protein of choice, such that it is suitable for microinjection and subsequent expression studies in live oocytes.
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Ex Vivo Stromal Cell Electroporation of Factor IX cDNA for Treatment of Hemophilia B
Hemophilia B is an X-linked genetic disorder that typically ...
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Electrical Recordings from the Accessory Olfactory Bulb in VNO-AOB Ex Vivo Preparations
Electrical recordings from individual accessory olfactory bu...
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