Preparation of Template DNA and Labeling Techniques

A variety of probes can be used for in situ hybridization, depending on the application and the labeling strategy. In general, RNA probes (riboprobes) are now more commonly used. However, some laboratories may still prefer to use DNA probes for in situ hybridization, and a number of techniques for this type of labeling have been described in this chapter. The preparation of plasmid DNA and the subsequent use of polymerase chain reaction products as labeling templates are discussed in detail. Both radioactive and nonradioactive labeling procedures are described and the latest nonradioactive detection methods are outlined.