Select Protocols of High-Throughput ee-Screening Systems for Assaying Enantioselective Enzymes

As delineated in Chapter 26 , the directed evolution of enantioselective enzymes for use in synthetic organic chemistry requires the availability of high-throughput screening systems for determining the enantiomeric excess (ee ) of thousands of samples (1 ). In this chapter the protocols of some of the most practical ee -screens are presented.