Login
欢迎浏览恩派尔生物资料网
我要投稿 请登录 免费注册 安全退出

您现在的位置是: 首页 > 实验方法 > 细胞技术

细胞技术

Quantifying Amplicons with ELISA

2025-02-11 细胞技术 加入收藏
Among the numerous assays proposed for quantifying specific nucleic-acid sequenc

Among the numerous assays proposed for quantifying specific nucleic-acid sequences in biological samples, PCR offers the greatest sensitivity and versatility. The assay for quantifying the amount of polymerase chain reaction (PCR) products is a crucial step in any quantitative PCR method. It should be sensitive and specific, able to display a wide dynamic range, nonradioactive, easy to do, and inexpensive. The results of the assay should also be easily digitalized. Quantification of amplicons with enzyme-linked immunosorbent assay (ELISA) fulfills these criteria. It can be automatized and readers are already available in most research and clinical laboratories. This assay can be accomplished by using colorimetry, fluorometry, or luminometry, depending on the substrate used. Luminometry displays the best sensitivity and has the widest dynamic range of these three methods (1and see Subheading 1.2.3. ). In this chapter, we will describe some of the available formats, the one we have been using this past few years, and its use in kinetic quantitative PCR or with internal standard.

文章底部广告位

文章评论

加载中~