Analysis of Chromatin Composition of Repetitive Sequences: The ChIP-Chop Assay
Chromatin immunoprecipitation (ChIP) is a powerful method that allows to probe specific protein-DNA interactions in vivo and to estimate the occupancy of proteins at specific sites of the genome. However, the traditional ChIP assay is not able to distinguish whether repeats that share identical sequences display a different composition of associated factors and, consequently, different functions. The ChIP-chop method provides a useful application to analyze the interaction of proteins with repetitive sequences based on their CpG methylation content. The detailed ChIP-chop protocol that serves to determine the chromatin composition of active and silent ribosomal RNA (rRNA) genes, repeats that share identical sequences but display distinct functions and chromatin compositions, is reported here.
- 上一篇
Review of Molecular Mechanisms Involved in the Activation of the Nrf2-ARE Signaling Pathway by Chemo
Human exposures to environmental toxicants have been associa...
- 下一篇
Identification of Genes Involved in Tumor-Like Properties of Embryonic Stem Cells
Embryonic stem (ES) cells are pluripotent stem cells derived...