Isolation and Short-Term Culture of Human Epidermal Langerhans Cells

The human epidermis consists of a heterogeneous population of cells including keratinocytes in various stages of differentiation, Merkel cells, melanocytes, and Langerhans’ cells (LC). The latter account for only around l–3% of the epidermal population, but they represent the major antigen-presenting cells of the epidermis (1 )