Login
欢迎浏览恩派尔生物资料网
我要投稿 请登录 免费注册 安全退出

您现在的位置是: 首页 > 实验方法 > 细胞技术

细胞技术

Homologous Gene Targeting to Study ECM Assembly

2025-02-22 细胞技术 加入收藏
Genetically engineered mutant strains of mice have become invaluable too to deci

Genetically engineered mutant strains of mice have become invaluable too to decipher complex biological processes, such as ECM assembly, and to dissect phenotypic overlaps, like the genodermatoses. Homologous gene targe ing in embryonic stem (ES) cells is increasingly favored over the transgen approach because it allows the study of mutations in homozygosity and without altering the remainder of the genome (1). Homologous gene targeting has been successfully employed to abrogate the expression or alter the structure of ECM-coding genes (2,3). In both cases, the experimental protocol consists of the following three steps: a. construction of the targeting vector; b. homologous recombination in ES cells; and c. generation of mutant mouse strains.

文章底部广告位

文章评论

加载中~