细胞组分和细胞器――细胞骨架
Fixation and Immunofluorescence of the Cytoskeleton (Mitchison Lab) Recycling
- Fixation and Immunofluorescence of the Cytoskeleton (Mitchison Lab)
- Recycling Tubulin (Mitchison Lab)
- Labeling Tubulin and Quantifying Labeling Stoichiometry (Mitchison Lab)
- Tubulin Polymerization with GTP/GMPCPP/Taxol (Mitchison Lab)
- Preparation of Segmented and Polarity Marked Microtubules (Mitchison Lab)
- Microtubule Spindowns for Visual Analysis (Mitchison Lab)
- Flow Cell Assays with Microtubules: Motility/Dynamics in Fluorescence and VE-DIC (Mitchison Lab)
- Negative Stain Electron Microscopy of Microtubules (Mitchison Lab)
- Microtubule Pelleting (Mitchison Lab)
- Actin - Chromatography Purification (The Cell Biology and Cytoskeleton Group, HMS)
- Actin - Labeling with Pyrene Iodoacetamide (The Cell Biology and Cytoskeleton Group, HMS)
- Actin Binding Assay with Rhodamine-Phalloidin (The Cell Biology and Cytoskeleton Group, HMS)
- Actin Extraction from Acetone Powder (The Cell Biology and Cytoskeleton Group, HMS)
- Actin Preparation - Acetone Powder Method (The Cell Biology and Cytoskeleton Group, HMS)
- Plasma Gelsolin Preparation (The Cell Biology and Cytoskeleton Group, HMS)
- Some Buffers for Actin Work (The Cell Biology and Cytoskeleton Group, HMS)
- Fixing and Immunostaining Cells (The Cell Biology and Cytoskeleton Group, HMS)
- Fixing and Staining Cells for Actin with Fluorescently-Labeled Phalloidin (The Cell Biology and Cytoskeleton Group, HMS)
- "Cells on Gels" (The Cell Biology and Cytoskeleton Group, HMS) this protocol describes method for culture cell on a thin polyacrylamide-based, collagen-coated flexible substrate. By maintaining a constant total concentration of acrylamide while usr/localying the concentration of bis-acrylamide, it's able to obtain a series of chemically identical substrates with a wide range of flexibility. By using imaging techniques, cells' response to differences in substrate flexibility can be detected.
- Immunofluorescent Localization of Tubulin (William H. Heidcamp) For cultured cell
- Isolation of Microtubules (Bovine Brain) (William H. Heidcamp)
- Viscosity & Polymerization of Microtubules (William H. Heidcamp)
- TEM Visualization of Microtubules (William H. Heidcamp)
- SDS Gel Electrophoresis of Tubulin\MAPs (William H. Heidcamp)
- Isolation of Actin and Myosin Filaments (William H. Heidcamp)
- Yeast Actin Capture Assay (D. Amberg)
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