Method for Conducting Microarray Study of Oxidative Stress Induced Gene Expression
Cellular systems produce reactive oxygen species during the process of metabolis
Cellular systems produce reactive oxygen species during the process of metabolism. Oxidative stress results in the activation or repression of many genes in important signaling pathways. DNA microarrays allow for a high throughput evaluation of the changes in gene expression levels in any biological system. In this study, we describe a method to employ gene expression microarrays to study the transcriptional changes in redox-engineered cell lines that will overexpress MnSOD and/or catalase in the mitochondria.
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Methods to Quantify microRNAs in the Myc Gene Network for Posttranscriptional Gene Repression
As a global transcription factor, Myc regulates both protein...
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Knockdown of Telomerase RNA Using Hammerhead Ribozymes and RNA Interference
More than 85% of human cancers and over 70% of immortalized ...