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细胞技术

Method for Conducting Microarray Study of Oxidative Stress Induced Gene Expression

2025-02-27 细胞技术 加入收藏
Cellular systems produce reactive oxygen species during the process of metabolis

Cellular systems produce reactive oxygen species during the process of metabolism. Oxidative stress results in the activation or repression of many genes in important signaling pathways. DNA microarrays allow for a high throughput evaluation of the changes in gene expression levels in any biological system. In this study, we describe a method to employ gene expression microarrays to study the transcriptional changes in redox-engineered cell lines that will overexpress MnSOD and/or catalase in the mitochondria.

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