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Isoelectric Focusing and Immunofixation of Monoclonal Antibodies

2025-02-28 细胞技术 加入收藏
Isoelectric focusing of monoclonal antibodies in agarose gel is one of several w

Isoelectric focusing of monoclonal antibodies in agarose gel is one of several ways to characterize a hybridoma. Isoelectric focusing is performed on each monoclonal antibody, and the mouse immunoglobulin bands are immobilized by soaking the gel with rabbit antimouse immunoglobulin before staining with Coomassie Blue.

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