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细胞技术

Exploring Functional In Vivo Consequences of the Selective Genetic Ablation of mTOR Signaling in T H

2025-03-01 细胞技术 加入收藏
The mammalian Target of Rapamycin (mTOR) defines a crucial link between nutrient

The mammalian Target of Rapamycin (mTOR) defines a crucial link between nutrient sensing and immune function. In CD4+ T cells, mTOR has been shown to play a critical role in regulating effector and regulatory T cell differentiation as well as the decision between full activation versus the induction of anergy. In this chapter, we describe how our group has employed the Cre-lox technology to genetically delete components of the mTOR signaling complex in T cells. This has enabled us to specifically interrogate mTOR function in T cells both in vitro and in vivo. We also describe techniques used to assay immune function and signaling in mTOR-deficient T cells at the single-cell level.

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