细胞技术

胚胎干细胞培养技术大全

2025-03-03 细胞技术加入收藏

MEDIA AND SOLUTIONS REQUIRED FOR ROUTINE ES CELL CULTURERoutine Culturing of ES

MEDIA AND SOLUTIONS REQUIRED FOR ROUTINE ES CELL CULTURE

Routine Culturing of ES Cells ISOLATION OF PRIMARY MOUSE EMBRYO FIBROBLASTS MITOMYCIN C TREATMENT OF PMEFs GAMMA IRRADIATION OF PMEF'S PMEF FEEDER LAYER CONCENTRATION ELECTROPORATION OF ES CELLS AND ISOLATION OF H/R CLONES KARYOTYPING ES CELLS VASECTOMY OF MALE MICE Normaski Optics MEDIA FOR EMBRYO CULTURE AND MANIPULATION Suppliers PREPARATION OF MICROINJECTION PIPETTES EMBRYO MANIPULATION PIPETTES (FLUSHERS ETC) COLLECTION OF MORULAE and Earlier Aggregation of ES cells and eight cell stage embryos BLASTOCYST TRANSFER Sending sera to testing for mouse pathogens. SUPEROVULATION OF MICE FLUSHING OUT OF BLASTOCYSTS Alizarin Red S (bone) and alcian blue (cartilagestaining of cleared skeletons. Routine Splitting and freezing of cells SOFT AGAR ASSAY FOR COLONY FORMATION VIRUS INFECTION OF FIBROBLASTS DNA TRANSFECTION OF EUKARYOTIC CELLS USING CALCIUM PHOSPHATE LIPOFECTAMINE FOR TRANSFORMATION OF EUKARYOTIC CELLS ELECTROPORATION OF COS-7 CELLS Growth and transfection of PC12 cells. CELL CULTURE FROM WHOLE MICE EMBRYO (DAY 10 - 11 PC) PCR GENOTYPING FROM the EMBRYO YOKE SACK b-gal staining of eukaryotic cells in vitro Comments on db-cAMP and forskolin Comments on Ro 31.8220 IMMUNOFLUORESENCE ON CHAMBER SLIDES PHOTOMICROGRAPHY Reagents:

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