RIPA裂解液配方
1ml RIPA buffer contains 35μl proteinase inhibitor(sigma cat no P8340) and 10μl
1ml RIPA buffer contains 35μl proteinase inhibitor(sigma cat no P8340) and 10μl phosphatase inhibitor
RIPA buffer 最终浓度
1M Tris-HCl pH7.5) 5 ml 50mM
NaCl 0.87g 0.15 M
Na-deoxycholale 0.1g (1%)
0.5M EDTA(pH8.0) 0.8ml
NaF 41 mg
Nonidet P40 1 ml (1%)
Aprotinin10μg/μl 100μl 10μg/ml
加水到 100ml
Aprotinin 10μg/μl ;用10mM HEPES-KOH(PH8.0)溶解,备用。