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Detecting DNA Methylation Using the Methylated CpG Island Amplification and Microarray Technique

2025-03-15 细胞技术 加入收藏
Methylated CpG island amplification and microarray (MCAM) is a two-color array t

Methylated CpG island amplification and microarray (MCAM) is a two-color array technique that quantifies DNA methylation by hybridizing equimolar amounts of treated vs. control cell-line DNA to an array platform. Sample preparation, hybridization, and scanning are performed over 1 week, but multiple samples can be prepared simultaneously, allowing for high-throughput processing and data acquisition. One microarray slide is required for each sample assayed, with a control sample hybridized with each test sample.

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