Generation and Application of Phospho-specific Antibodies for p53 and pRB
The functions of many proteins are likely to be regulated by phosphorylation. Th
The functions of many proteins are likely to be regulated by phosphorylation. Thus, antibodies that can recognize specifically phosphorylated sites on proteins have a wide variety of uses for studying the function and regulation of phosphoproteins. We have improved methods for generation of phosphorylation site-specific antibodies and have successfully obtained antibodies for the analysis of most of the phosphorylation sites on p53 and RB proteins.