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细胞技术

Coimmunoprecipitation Assay for the Detection of Kinase-Substrate Interactions

2025-03-17 细胞技术 加入收藏
Coimmunoprecipitation is a powerful tool to study protein-protein interactions a

Coimmunoprecipitation is a powerful tool to study protein-protein interactions and can be used to test for the physical association between a known protein kinase and its substrate. In this chapter, the author describes a protocol for the preparation of a cell lysate, the immunoprecipitation of the antigen, and the analysis of the immune complex by gel electrophoresis and immunoblotting with antibodies that recognize the putative associated protein.

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