Multiplex Caspase Activity and Cytotoxicity Assays
Multiplexed assay chemistries provide for multiple measurements of cellular parameters within a single assay well. This experimental practice not only is more cost efficient but provides more informational content about a compound or treatment. For instance, multiplexed caspase activity assays can help establish the kinetics and magnitude of initiator and effector caspase induction by candidate compounds or treatments. The ability to combine the activity profiles within the same sample provides a level of normalization not possible with parallel assays. Furthermore, multiplexing caspase activity assays with viability and/or cytotoxicity assays can support conclusions regarding cytotoxic mechanism and provide normalization that may help correct for differences in cell number.
- 上一篇
Mixture-Based Peptide Libraries for Identifying Protease Cleavage Motifs
All proteases and peptidases are to some extent sequence-spe...
- 下一篇
Quantification of Cell Motility: Gold Colloidal Phagokinetic Track Assay and Wound Healing Assay
Cellular migration is an integral aspect in response to extr...