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细胞技术

Spheroids of Prostate Tumor Cell Lines

2025-03-17 细胞技术 加入收藏
Sutherland and coworkers developed and used the spheroid model in the 1970s for

Sutherland and coworkers developed and used the spheroid model in the 1970s for radiobiological studies (1 –6 ). Spheroids are three-dimensional (spherical) clusters of tumor cells grown from one or several cell clones. The spheroid model contains many of the elements of a tumor xenograft including an extracellular matrix and cell-cell/cell-matrix interactions (7 –10 ); a spatial geometry that can produce well-defined drug and nutrient concentration gradients (11 ,12 ); subpopulations of cells that are quiescent, hypoxic, necrotic, or near-necrotic (13 ); and the potential to monitor response over a protracted time-period after different treatment schedules (14 ). These similarities to a tumor xenograft are coupled with many of the experimental advantages of a monolayer culture including the ability to rigorously control experimental and treatment conditions, the ability to investigate response and study mechanisms, and also the ability to investigate a large number of agents and combinations rapidly and at low cost.

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