Differential Screening of cDNA Libraries for Analysis of Gene Expression During Tumor Progression
The ability to examine gene expression differences during cancer progression is crucial to our understanding of the biological events that lead to uncontrolled growth of malignant cells. Differential expression screening of complementary DNA libraries is described in detail here as a valuable method for monitoring gene expression changes in a prostate cancer xenograft model. Using Southern blot analysis of complementary DNA clones and Northern blot analysis to confirm differential expression, several androgen-regulated transcripts have been identified that may be important in prostate cancer progression.
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Alterations in Oncogenes, Tumor Suppressor Genes, and Growth Factors Associated with Epithelial Ovar
More than 90% of epithelial ovarian cancers are clonal neopl...
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Detection of HER2 Gene Amplification by Fluorescence In Situ Hybridization in Breast Cancer
Fluorescence in situ hybridization (FISH) is increasingly re...