Kinetworks Protein Kinase Multiblot Analysis
The proteomics analysis of protein kinases and other cell-signaling proteins in tumor samples by traditional two-dimensional (2-D) gel electrophoresis is complicated by the low abundance of these regulatory proteins relative to metabolic enzymes and structural proteins. We present an antibody-based method called Kinetworks™ that relies on sodium dodecyl sulfate (SDS)-polyacrylamide minigel electrophoresis and multilane immunoblotters to permit the specific and quantitative detection of 45 or more protein kinases or other signal transduction proteins at once. The technique can also permit the resolution of these proteins based on differences in their phosphorylation state and other forms of covalent modification. Kinetworks™ profiling of protein kinases in solid human tumors and cell lines can reveal profound differences in their expression and phosphorylation states, which can serve for the identification of cancer diagnostic markers and therapeutic targets for drug discovery.
- 上一篇
Organizational Issues in Providing High-Quality Human Tissues and Clinical Information for the Suppo
Superior-quality human tissues are required to support many ...
- 下一篇
Lymphatic Invasion as a Prognostic Biomarker in Primary Cutaneous Melanoma
Melanoma has a propensity for lymph node metastasis. However...