Login
欢迎浏览恩派尔生物资料网
我要投稿 请登录 免费注册 安全退出

您现在的位置是: 首页 > 实验方法 > 细胞技术

细胞技术

Production of Antibody Fragments in a Bioreactor

2025-03-20 细胞技术 加入收藏
Clinical use of antibodies or antibody fragments is dependent on highly effectiv

Clinical use of antibodies or antibody fragments is dependent on highly effective production systems. Antibody fragments like single-chain Fv or Fab have been successfully expressed in microorganisms for more than a decade, and the most commonly used host has been Escherichia coli . The ease of genetic manipulation in E. coli has made it the first choice for expression of antibody fragments, and by fusion of the protein to a signal sequence, antibody fragments are secreted to the periplasm of the bacteria, allowing correct folding and recovery of functionally active proteins. Finally, E. coli can be grown to high cell densities in an inexpensive medium allowing high volumetric yield of antibody fragments.

文章底部广告位

文章评论

加载中~