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Extraction and Purification of Plasmid DNA

2025-03-25 生物化学 加入收藏
Plasmids may be isolated by a variety of methods many of which rely on the diffe

Plasmids may be isolated by a variety of methods many of which rely on the differential denaturation and reannealing of plasmid DNA compared to chromosomal DNA. Many of these are rapid, small-scale “minipreps” that may be used effectively for plasmid analysis and further manipulation. The methods differ primarily in the means by which cells are lysed.

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