Polyacrylamide Gel Electrophoresis of Fluorophore-Labeled Carbohydrates from Glycoproteins
Prior to 1980, most methods for analysis of glycoprotein carbohydrates utilized column, thin-layer, and paper chromatography, gas chromatography, mass spectroscopy, and rarely nuclear magnetic resonance spectroscopy. These methods required relatively large amounts of materials (micromoles), specialized training and experience, and in some cases, significant capital equipment outlays. Because of these restrictions, convenient carbohydrate analysts on small samples was not available to most biologists. Recently, improvements in chromatographic methods, labeling methods for carbohydrates, carbohydrate-specific enzymes, and higher resolution electrophoresis methods have allowed carbohydrate analysts to be done on nanomolar amounts of material. Because of these improvements, today’s biologist now has an improved ability to evaluate the role of carbohydrates in their research and development work.
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SPR Biosensor as a Tool for Screening Prion Protein Binders as Potential Antiprion Leads
Prion diseases, also called transmissible spongiform encepha...
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Screening cDNA Libraries by Hybridization with Double-Stranded DNA Probes and Oligonucleotides
Probably the most commonly used method to screen a cDNA libr...