Login
欢迎浏览恩派尔生物资料网
我要投稿 请登录 免费注册 安全退出

您现在的位置是: 首页 > 实验方法 > 生物化学

生物化学

Plasmid Preparation on Sephacryl S1000

2025-03-25 生物化学 加入收藏
Production of pure plasmid DNA is a prerequisite for most laboratories engaged i

Production of pure plasmid DNA is a prerequisite for most laboratories engaged in recombinant DNA research. There are a number of procedures available for plasmid purification, all of which have common features; namely, (1 ) growth of plasmid-containing bacteria, (2 ) lysis of bacteria, (3 ) low-speed centrifugation to remove the majority of bacterial DNA and protein, (4) an ultracentrifugation step in cesium chloride and ethidium bromide to separate plasmid DNA from RNA and chromosomal DNA, and (5) recovery of plasmid DNA band (extraction of ethidium bromide and cesium chloride). Steps (4) and (5) of this procedure are expensive, time-consuming, and require expensive machinery [ultracentrifuge, rotor(s)]. In addition, incomplete extraction of ethidium bromide can result in plasmid DNA that is not manipulable by many enzymes commonly used in DNA research.

文章底部广告位

文章评论

加载中~