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Dealing with different methods for Kluyveromyces lactis -galactosidase purification

2025-03-27 生物化学 加入收藏
Several micro-scale chromatography-based procedures for purification of the β-ga

Several micro-scale chromatography-based procedures for purification of the β-galactosidase from the yeast Kluyveromyces lactis were assayed. Purified enzyme was suitable to be used as antigen to induce polyclonal antibodies production. Specific staining of non-denaturing PAGE gels with chromogenic substrates allowed the determination of the number of subunits forming the native enzyme.

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