Precise large deletions by the PCR-based overlap extension method
The authors describe an efficient method for generating large deletions (>200
The authors describe an efficient method for generating large deletions (>200 nts) of precise length using the PCR-based method of gene splicing by overlap extension (1 ). This method is technically simple and less time consuming than conventional loop-out mutagenesis techniques requiring preparation of a single-stranded DNA template.