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Precise large deletions by the PCR-based overlap extension method

2025-03-27 生物化学 加入收藏
The authors describe an efficient method for generating large deletions (>200

The authors describe an efficient method for generating large deletions (>200 nts) of precise length using the PCR-based method of gene splicing by overlap extension (1 ). This method is technically simple and less time consuming than conventional loop-out mutagenesis techniques requiring preparation of a single-stranded DNA template.

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