Sequencing DNA Fragments Cloned into M13 and Phagemid Vectors
The dideoxy chain-termination method (1 ) involves enzymatic elongation of a oli
The dideoxy chain-termination method (1 ) involves enzymatic elongation of a oligonucleotide primer that is annealed to a single-stranded DNA template. Single-stranded DNA of bacteriophage M13 (2 ) or phagemid vectors (3 ) give the most consistently satisfactory sequencing data. However, denatured double-stranded plasmid DNA can also serve as a suitable template and can also yield several hundred nucleotides of sequence information per reaction.
- 上一篇
Amino Acid Profiling for the Diagnosis of Inborn Errors of Metabolism
The diagnosis of inherited metabolic disorders of amino acid...
- 下一篇
Synthesis of (2R, 3R, 4R, 5S)-5-tert-Butyloxycarbonylamino-3,4-Dihydroxy-2-1sopropyl-3,4-O,O-Isoprop
The dipeptidic dihydroxyethylene isostere (27, 3R , 4R , 5S ...