Screening of Pirkle-Type Chiral Stationary Phases for HPLC Enantioseparations

The majority of enantiomeric separations for purity analysis and quality control continue to be performed by normal-phase liquid chromatography and supercritical fluid chromatography. In this chapter, representative chromatographic screening procedures for the enantioseparations using Pirkle-type stationary phases are presented. As Pirkle-type phases are commonly applied to the preparative chromatographic isolation of enantiomers, volatile modifiers are used in this screen in order to be subsequently compatible with the techniques used to recover analytes from preparative scale isolations. The Stage 1 screen presented here is used initially for screening chiral entities. The gradients use cyclohexane and ethanol both with and without chromatographic modifiers. The Stage 2 screen is used for more challenging to resolve compounds that do not exhibit resolution using the Stage 1 screening procedure.