Synthesis of DNA Mimics Representing HypNA-pPNA Hetero-Oligomers
The methods for the synthesis and purification of negatively charged peptide nuc
The methods for the synthesis and purification of negatively charged peptide nucleic acid (PNA)-relative deoxyribonucleic acid (DNA) mimics containing alternating residues of phosphono peptide nucleic acid (pPNA) monomers and PNA-like monomers on the base of trans -4-hydroxy-L -proline are described. Examples of the chimeric oligomers hybridization with complementary DNA and ribonucleic acid fragments are demonstrated.
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PFGE Using Double-Inhomogeneous Fields or Orthogonal Field-Alternating Gel Electrophoresis (OFAGE)
The methods discussed in this chapter were the first pulsed-...
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Targeting mRNAs by Engineered Sequence-Specific RNase P Ribozymes
The methods of using engineered RNase P catalytic RNA (terme...
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