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Detection of Lipid Hydroperoxide-Derived Protein Modification with Polyclonal Antibodies

2025-03-29 生物化学 加入收藏
The oxidation of lipids can cause the formation of lipid hydroperoxide followed

The oxidation of lipids can cause the formation of lipid hydroperoxide followed by the degradation of the peroxide. The formed lipid-decomposition products such as aldehydes can easily react with biomolecules such as proteins (1 ). The aldehyde-derived adduct formation has been examined by many researchers using chemical and immunochemical methods (1 -3 ). However, on the other hand, the lipid hydroperoxide itself might react with protein as already suggested (4 ). We hypothesize that the lipid hydroperoxide-derived modification occurs and specific products derived from the reaction should be formed. In this chapter, we describe the methodology of the preparation of polyclonal antibodies (PAbs) to lipid hydroperoxide-modified proteins. We also show the preparation of the polyclonal antibody to Nε -(hexanoyl)lysine, which is identified as a novel lipid-lysine adduct from the reaction mixture of linoleic acid hydroperoxide and lysine (5 ).

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