HPLC of DNA and PCR Products
The polymerase chain reaction (PCR) has rapidly become a standard laboratory tec
The polymerase chain reaction (PCR) has rapidly become a standard laboratory technique. With the continuous development of PCR technology, there has been a growing need for PCR product quantitation. High-performance liquid chromatography (HPLC) is well accepted as a quantitative technique in many diverse applications areas because the technique can provide reliable, precise, and sensitive sample detection, and wide dynamic range.