Login
欢迎浏览恩派尔生物资料网
我要投稿 请登录 免费注册 安全退出

您现在的位置是: 首页 > 实验方法 > 生物化学

生物化学

Rapid (Ligase-Free) Subcloning of Polymerase Chain Reaction Products

2025-03-29 生物化学 加入收藏
The polymerase chain reaction (PCR) is a versatile, widely used method for the p

The polymerase chain reaction (PCR) is a versatile, widely used method for the production of a very large number of copies of a specific DNA molecule (1 ,2 ). For some applications, it is advantageous to subclone the PCR product into a plasmid vector for subsequent replication in bacteria (3 –6 ). Subcloning the PCR product into a plasmid vector has several advantages: The amplified fragment can be sequenced with greater reliability, only one allele is sequenced per clone, and the vector containing the PCR product may be used for other molecular biological experiments, e.g., in vitro transcription, transfection, and further amplification in bacteria.

文章底部广告位

文章评论

加载中~