Systems for the Cell-Free Synthesis of Proteins
We describe a system for the cell-free expression of proteins based on extracts from Escherichia coli. Two reaction configurations, batch and continuous exchange, are discussed and analytical scale as well as preparative scale setups are documented. Guidelines for the systematic development and optimization of cell-free expression protocols are given in detail. We further provide specific protocols and parameters for the cell-free production of membrane proteins. High-throughput screening applications of CF expression systems are exemplified as new tools for genomics and proteomics studies.
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Semiquantitative RT-PCR analysis to assess the expression levels of multiple transcripts from the sa
We describe a semiquantitative RT-PCR protocol optimized in ...
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Quantum Dot Hybrid Gel Blotting: A Technique for Identifying Quantum Dot-Protein/Protein-Protein Int
We describe an alternative to the molecular biology techniqu...