Analysis of Rho GTPase Function in Axon Pathfinding Using Caenorhabditis elegans

We provide information and protocols for the analysis of Rho GTPase function in axon pathfinding in Caenorhabditis elegans . The powerful molecular, genetic, imaging, and transgenic tools available in C. elegans make it an excellent system in which to study the in vivo roles of Rho GTPases. Methods for imaging of axon morphology in Rho GTPase single and double mutants are provided, as well as methods for the construction of transgenic C. elegans strains carrying exogenously introduced transgenes that drive the expression of constitutively active and dominant negative mutants.