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Diet, Epigenetics, and Cancer
Cancer encompasses a highly heterogeneous group of diseases. It has been thought
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Interaction of p53 with Cellular Proteins
Cellular proteins that interact with p53 play a major role in both positive and
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有限稀释法
有限稀释法的程序①制备饲养细胞悬液②转染了重组质粒后的细胞计数,并调细胞数在1~5103/ml③取130个细胞放入6.5ml含饲养细胞完全培养液,即20个细胞/
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Enhanced Permeability and Retention (EPR) Effect for Anticancer Nanomedicine Drug Targeting
Effective cancer therapy remains one of the most challenging tasks to the scient
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Multicolor Spectral Karyotyping in Multiple Myeloma
Multiple myeloma is a plasma cell disorder characterized at the cytogenetic leve
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luciferase检测的标准品问题
荧光素酶作为一种报告基因要在检测时比较其相对发光强度值,就要在转染时引入另外一个报告基因进行转染条件、效率的标准化归一。Promega公司在这方面有双荧光素酶检
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吉凯基因分享:结直肠癌研究体内外模型综述
引言:上海吉凯基因化学技术有限公司成立于 2002 年,拥有国内最大的慢病毒文库,同时也是国内最大的疾病关键基因科研服务供应商,在疾病研究领域的实验积累已逾 1
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MicroRNA Biogenesis and Cancer
MicroRNAs are short non-coding RNA molecules that are involved in diverse physio
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Southern Blotting of IgH Rearrangements in B-Cell Disorders
Southern blotting is a method whereby DNA fragments in the gel are denatured by
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Human Solid Tumors: Cytogenetic Techniques
The field of cytogenetics has had a great impact on many aspects of medical and
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细胞组分的分析方法
一.基本原理核酸分子杂交技术是目前分子生生物学、细胞生物学和生物化学研究中应用最广泛的技术之一,是定性、定量和定位检测两条来源不同的聚核苷酸链上碱基顺序同源性的
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Assays for Cyclin-Dependent Kinase Inhibitors
Cyclin-dependent kinases (CDK) are serine/threonine kinases that regulate cell c
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单克隆抗体制备-液体石蜡的处理
问:单克隆抗体制备过程中在往BALB/C小鼠腹腔折注射的液体石蜡要如何处理?要高压吗?先谢谢各位!答:液体石蜡不用高压,BALB/C小鼠腹腔注射0.5-1毫升,
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肿瘤细胞侵袭试验(Tumour Invasion Assay)
一、原理Matrigel 是从小鼠EHS肉瘤中提取的基质成分,含有LN、IV型胶原、接触蛋白和肝素硫酸多糖,铺在无聚乙烯吡硌烷酮的聚碳酸酯滤膜上,能在DMEM培
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A Scientific Basis for Cancer Prevention: Defining the Role of Individual Cytosolic GST Isozyme
Highly electrophilic functional groups of exogenous and endogenous chemicals rep