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Proteomic Analysis of Protein Complexes in Toll-Like Receptor Biology
Purification of protein complexes and identification of the constituent componen
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Proteomics and Laser Microdissection
Two-dimensional gel electrophoresis (2-DE) combined with protein identification
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Protocol for Diversion of a CD4+Response to theT-Helper 2 Cell Pathway
Differentiated CD4+ T-cells produce a restricted set of cytokines, allowing thei
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Protocol for Limiting Dilutions
Protocol for Limiting DilutionsNOTE: The sooner after cell fusion you can do a l
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Protocols for Identifying, Enumerating, and Assessing Mouse Eosinophils
Eosinophils are prominent in allergic diseases, and their effector functions are
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Protocols for the Induction and Evaluation of Systemic Anaphylaxis in Mice
Mouse models of systemic anaphylaxis are important tools for the study of mast c
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Proviral Load Determination of HTLV-1 and HTLV-2 in Patients Peripheral Blood Mononuclear Cells by R
TaqMan real-time PCR assays were developed to determine the proviral load (PVL)
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Psychoneuroimmunology: The Experiential Dimension
Accumulating evidence has made clear that experience—the knowledge an individual
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Psychosocial Job Stress and Immunity: A Systematic Review
The purpose of this review was to provide current knowledge about the possible a
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Pulmonary Antigen Presenting Cells: Isolation, Purification, and Culture
Antigen presenting cells (APCs) such as dendritic cells (DCs) and macrophages co
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PulseChase Analysis for Studies of MHC Class II Biosynthesis, Maturation, and Peptide Loading
Pulse–chase analysis is a commonly used technique for studying the synthesis, pr
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Pulse-field Gel Electrophoresis for Epidemiological Studies of Streptococcus pneumoniae
Electrophoresis techniques have allowed for very effective separation of nucleic
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Purification and Analysis of Strep-tagged Antibody-Fragments
The Strep-tag is a nine-residue minimal peptide sequence with intrinsic affinity
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Purification and Characterization of His-Tagged Antibody Fragments
Immobilized metal affinity chromatography (IMAC) has become a widely used method
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Purification and Conjugation of Type 1 Ribosome-Inactivating Proteins
Ribosome-inactivating proteins (RIPs, 1 ) are plant enzymes that damage ribosome