-
Purification of Complement Components, Regulators, and Receptors by Classical Methods
The aim of this chapter is to describe methods for purification of the individua
-
Purification of DNA Topoisomerases and Inhibition by Fluoroquinolones
Fluoroquinolones act by inhibiting the essential type II topoisomerases, DNA gyr
-
Purification of Glycoproteins and Their Use as Immunogens
Glycosylation is a major posttranslational modification that produces heterogene
-
Purification of Human Complement Protein C5
Complement C5 is cleaved by proteolysis in the terminal phase of complement acti
-
Purification of Human Monoclonal Antibodies and Their Fragments
This chapter summarizes the most common chromatographic mAb and mAb fragment pur
-
Purification of Human NK Cell Developmental Intermediates from Lymph Nodes and Tonsils
Accumulating data indicate that human natural killer (NK) cells undergo terminal
-
Purification of Immature CD4+CD8+ Thymocytes by Panning with Anti-CD8 Antibody
Most T lymphocytes of the immune system differentiate within the thymus along th
-
Purification of Immunoglobulin G (IgG)
Several immunological procedures can be successfully carried out using nonpurifi
-
Purification of Large Cytosolic Proteases for In Vitro Assays: 20S and 26S Proteasomes
Proteasomes are the main cytosolic proteases responsible for generating peptides
-
Purification of mAb (IgG)
1. Materials(1) Antibody 7E3, 2L sup grown in flasks, frozen and thawed overnigh
-
Purification of Monoclonal Antibodies
Hybridoma technology has made possible the production of specific homogeneous an
-
Purification of Monoclonal Antibodies Using Protein A/G
A major breakthrough in immunology came with the discovery that large amounts of
-
Purification of Native Surfactant Protein SP-A from Pooled Amniotic Fluid and Bronchoalveolar Lavage
Surfactant protein SP-A is a hydrophilic glycoprotein, similar to SP-D, which pl
-
Purification of Recombinant Chemokines from E. coli
The majority of chemokines are highly basic, small proteins, with a molecular ma
-
Purification of Reduced and Alkylated Antibody Subunits
Antigen-binding sites in antibodies are formed by the variable regions of light
