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PCR Cloning of Human Immunoglobulin Genes
One of the first steps in an antibody-engineering project is the isolation of th
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DNA Sequencing by Capillary Array Electrophoresis
On May 9,1998, PE Biosystems announced that they would introduce a new instrumen
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Mannose-6-Phosphate: A Regulator of LLO Destruction
Oligosaccharyltransferase (OT) catalyzes the signature reaction of the asparagin
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Base-Modified Oligonucleotides With Increased Duplex Stability: Pyrazolo[3,4-d] Pyrimidines Replacin
Oligonucleotides incorporating 8-aza-7-dazapurines (pyrazolo[3,4-d]pyrimidines)
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Hybridization of Fluorescein-Labeled Oligonucleotide Probes and Enhanced Chemiluminescence Detection
Oligonucleotide synthesizers are now commonplace with the result that oligonucle
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Synthesis and Purification of Phosphorodithioate DNA
Oligonucleotide analogs bearing modified phosphodiester linkages have been the f
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Production of DNA Hybridization Probes with Digoxigenin-Modified Nucleotides by Random Hexanucleotid
Oligolabeling using random hexanucleotide primers (1 ) is an effective method fo
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Manual Oligonucleotide Synthesis Using the Phosphoramidite Method
Oligodeoxyribonucleotides may be synthesized on a solid support, which allows fo
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Multiplex Polymerase Chain Reaction
Often, studies require the polymerase chain reaction (PCR) amplification of seve
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DNA Profiling: Theory and Practice
Of the vast range of techniques in use in modern molecular biology, DNA protilin
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Cyclic Guanosine 5-Monophosphate Binding to Regulatory GAF Domains of Photoreceptor Phosphodiesteras
Of the 11 families of mammalian cyclic nucleotide phosphodiesterases (PDEs), 5 c
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Analysis of cGMP Signaling in Adipocytes
Obesity has reached pandemic dimensions with more than half a billion adults aff
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Synthesis of O-Glycopeptides and Construction of Glycopeptide Microarrays
O -Glycosylation of proteins is an important modification which affects biologic
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Study of GPCRProtein Interactions Using Gel Overlay Assays and Glutathione-S-Transferase-Fusion Prot
Numerous recent studies have suggested that the predicted cytosolic domains of G
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Quantification of Endogenous Retinoids
Numerous physiological processes require retinoids, including development, nervo
