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Mass Spectrometric Determination of Protein Ubiquitination
Mass spectrometric methods of determining protein ubiquitination are described.
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DNA Transfection of Mammalian Cells Using Polybrene
Many techniques have been developed to transfect mammalian cells with DNA (1 –2
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Detection and Immobilization of Proteins Containing the 6xHis Tag
Many systems have been developed for the heterologous expression of recombinant
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Hybrid-Release Translation
Many situations arise in recombinant DNA research in which it is necessary to id
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Fluorescent Labeling of Proteins
Many single-molecule experimental techniques exploit fluorescence as a tool to i
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Microinjection of myo-Inositol(1,4,5)trisphosphate and Other Calcium-Mobilizing Agents into Intact A
Many receptor tyrosine kinases and seven-transmembrane receptors are directly co
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Measurement of Isofurans by Gas Chromatography-Mass Spectrometry/Negative Ion Chemical Ionization
Many methods have been developed to assess oxidative stress status in vivo, whic
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Isolating Chromosomal DNA from Bacteria
Many methods have been described for isolating DNA from prokaryotic cells. The c
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2-D Databases on the World Wide Web
Many laboratories have identified proteins on two-dimensional (2-D) maps, and ha
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Nonradioactive Labeling of DNA
Many important advances in molecular biology would not have been possible wlthou
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Capillary Affinity Gel Electrophoresis
Many human genetic diseases are caused by small alterations in DNA sequence of s
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Searching the Protein Interaction Space Through the MINT Database
Many fundamental processes involve protein–protein interactions. Recent advances
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Purification of Yeast Membranes and Organelles by Sucrose Density Gradient Centrifugation
Many experiments require isolation and purification of membranes and organelles
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Assays for RING Family Ubiquitin Ligases
Many eukaryotic proteins are regulated by the covalent attachment of ubiquitin o
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Pro-Oxidant and Antioxidant Effects of Estrogens
Many compounds of great structural diversity possess varying degrees of estrogen
