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Molecular Modeling of Lipase Binding to a SubstrateWater Interface
Interactions of lipases with hydrophobic substrate–water interfaces are of great
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T cell integrin overexpression as a model of murine autoimmunity
Integrin adhesion molecules have important adhesion and signaling functions. The
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Membrane Protein Insertion in E. coli
Integral membrane proteins typically span the lipid bilayer with hydrophobic α h
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Studying Antibody Conformations by Ultracentrifugation and Hydrodynamic Modeling
Intact immunologically active antibodies are too large for high-resolution struc
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Human Antibody Production Using Insect-Cell Expression Systems
Insect-cell expression systems provide a reliable and effective means for genera
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Continuous Fluorescent Monitoring of Cellular Calcium Fluxe: A Novel Perfusion System for the Invest
Inositol(1,4,5)trisphosphate [Ins(1,4,5)P3 ] is a well-characterized second mess
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Inositol Lipid-Dependent Functions in Saccharomyces cerevisiae: Analysis of Phosphatidylinositol Pho
Inositol phospholipids regulate many cellular processes, including cell survival
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Single-Cell Imaging Techniques for the Real-Time Detection of IP3 in Live Cells
Inositol 1,4,5-trisphosphate (IP3 ) is a ubiquitous second messenger, derived fr
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Proteasome Inhibitors in Cancer Therapy
Inhibitors of the proteasome have long been used in studies of protein turnover,
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Direct In-Gel Hybridization of DNA With Digoxigenin-Labeled Probes
In-gel hybridization with digoxigenin (DIG)-labeled probes has been shown to det
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Detection of DNA Hybridization Using Induced Fluorescence Resonance Energy Transfer
Induced fluorescence resonance energy transfer (iFRET) is a variation of resonan
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Measurement of Phosphoinositols and Phosphoinositides Using Radio High-Performance Liquid Chromatogr
Individual phosphoinositols and phosphoinositides in tissues and cells are commo
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Glycoprotein Characterization
Increasing numbers of studies are reporting the modification of prokaryotic prot
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Single Cell and Subcellular Measurements of Intracellular Ca2+ Concentration
Increases in bulk average cytoplasmic Ca2+ concentration ([Ca2+ ]c ) are derived
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Extending the Scope of Site-Specific Cysteine Bioconjugation by Appending a Prelabeled Cysteine Tag
Incorporating synthetic probes site-specifically into proteins is of central int
