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Single Cell and Subcellular Measurements of Intracellular Ca2+ Concentration
Increases in bulk average cytoplasmic Ca2+ concentration ([Ca2+ ]c ) are derived
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Extending the Scope of Site-Specific Cysteine Bioconjugation by Appending a Prelabeled Cysteine Tag
Incorporating synthetic probes site-specifically into proteins is of central int
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Site-Directed Mutagenesis Using Double-Stranded Plasmid DNA Templates
In vitro site-directed mutagenesis is an invaluable technique for studying prote
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In Vitro Selection Procedures for Identifying DNA and RNA Aptamers Targeted to Nucleic Acids and Pro
In vitro selection or systematic evolution of ligands by exponential enrichment
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Isolation of Rat Hepatocytes
In vitro models, based on liver cells or tissues, are indispensable in the early
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In Vitro UDP-Sugar:Undecaprenyl-Phosphate Sugar-1-Phosphate Transferase Assay and Product Detection
In vitro assays are invaluable for the biochemical characterization of UDP-sugar
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In vitro Sphingomyelinase Assay
In vitro Sphingomyelinase Assay Contributor: Suprya JayadevDate: May 25, 1993Rea
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Generation of gene deletions and gene replacements in Escherichia coli O157:H7 using a temperature s
In this work we describe protocols for the generation of gene deletions and gene
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The method of contact angle measurements and estimation of work of adhesion in bioleaching of metals
In this paper, we present our method for the measurement of contact angles on th
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Preparation and Dynamic Patterning of Supported Lipid Membranes Mimicking Cell Membranes
In this chapter, we describe standardized protocols for the self-assembly of sup
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Heterologous Expression of GPCRs in Fission Yeast
In this chapter, we describe methods to heterologously express G protein-coupled
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Identification of Efficient Cleavage Sites in Long-Target RNAs
In this chapter, we describe a procedure for identification of efficient hammerh
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Ubiquitination of GPCRs
In this chapter, we describe a method for detecting the ubiquitination status of
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MALDI-MS Data Analysis for Disease Biomarker Discovery
In this chapter, we address the issue of matrix-assisted laser desorption/ioniza
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Fluorescent DNAzyme Biosensors for Metal Ions Based on Catalytic Molecular Beacons
In this chapter, methods for designing metal ion sensors using fluorophore- and
