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Development of a new protocol for 2-day generation of mature dendritic cells from human monocytes
We developed a new 2-day protocol for the generation of dendritic cells (DCs) fr
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Fabrication of Polydimethylsiloxane Microfluidics Using SU-8 Molds
We detail the widely prevalent technique of polydimethylsiloxane (PDMS) molding
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Force and compliance measurements on living cells using atomic force microscopy (AFM)
We describe the use of atomic force microscopy (AFM) in studies of cell adhesion
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Biosensors Based on Carbon Nanotube-Network Field-Effect Transistors
We describe in detail the different steps involved in the construction of a carb
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Purification and Proteomic Analysis of a Nuclear-Insoluble Protein Fraction
We describe here a method for analyzing a rat liver nuclear-insoluble protein fr
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An improved method for constructing and selectively silanizing double-barreled, neutral liquid-carri
We describe an improved, efficient and reliable method for the vapour-phase sila
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An antibody-lectin sandwich assay for quantifying protein glycoforms
We describe an antibody-lectin sandwich assay for quantitation of glycoforms of
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Quantum Dot Hybrid Gel Blotting: A Technique for Identifying Quantum Dot-Protein/Protein-Protein Int
We describe an alternative to the molecular biology technique of polyacrylamide
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Systems for the Cell-Free Synthesis of Proteins
We describe a system for the cell-free expression of proteins based on extracts
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Semiquantitative RT-PCR analysis to assess the expression levels of multiple transcripts from the sa
We describe a semiquantitative RT-PCR protocol optimized in our laboratory to ex
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Quantitative Proteomics of Mouse Brain and Specific Protein-Interaction Studies Using Stable Isotope
We describe a new method for quantitative tissue proteomics using culture-derive
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Protein Processing Characterized by a Gel-Free Proteomics Approach
We describe a method for the specific isolation of representative N-terminal pep
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A Nonradioactive Assay for Nitric Oxide Synthase Activity in Tissue Extracts
We describe a fluorescence assay for nitric oxide synthase activity based on a n
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Quantification of Proteins and Metabolites by Mass Spectrometry Without Isotopic Labeling
We demonstrate the quantification capability and robustness of a new integrated
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In Vitro O-Antigen Ligase Assay
WaaL is a membrane enzyme that catalyzes the glycosidic bonding of a sugar at th
