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Yeast Two-Hybrid Library Screening
The two-hybrid system was originally devised by Fields and Song as a protein int
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Genome-Wide Analysis of Membrane Transport Using Yeast Knockout Arrays
The transport of membrane-bound proteins through post-Golgi compartments depends
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Inserting and Manipulating DNA in a Nanopore with Optical Tweezers
The translocation of small molecules and polymers is an integral process for the
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Gene Transfer and Expression in Tissue Culture Cells of Higher Eukaryotes
The transfer or “transfectron” of genetic material into tissue-culture cell line
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Profiling RNA Polymerase II Using the Fast Chromatin Immunoprecipitation Method
The traditional method for determining the transcription rate of a gene, nuclear
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Resin Technologies: Construction and Staining of Resin TMAs
The traditional formaldehyde-fixed paraffin-embedded tissue, and therefore the t
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Affinity-Capture and Solid-Phase Sequencing of Biotinylated Polymerase Chain Reaction Products
The time and labor required for generating nucleotide sequence information has b
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Detection of ERK1/2 Activities Using Affinity Reagents
The three major mammalian mitogen-activated protein kinase (MAPK) subfamilies in
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Fluorometric and Colorimetric Assessment of Thiobarbituric Acid-Reactive Lipid Aldehydes in Biologic
The thiobarbituric acid (TBA) assay was developed to quantitatively determine li
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On-Line Preconcentration and Separation of Antisense Oligonucleotides by ITP-CE in Dextran-Filled Ca
The therapeutic use of “antisense oligonucleotides” is a new approach to rationa
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The Isolation of Minicells
The term minicell was introduced by Adler et al. (1 ) for the small spherical ce
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Techniques for Determining the Metabolic Pathways of Eicosanoids and for Evaluating the Rate-Control
The term eicosanoid is derived from the chemical name for arachidonic acid, eico
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Scale-Up of Natural Products Isolation
The term “scale-up” has been interpreted here as increasing the output of the pr
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Gene Isolation by Exon Trapping
The technology of exon trapping, sometimes called exon amplification, strives to
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Analysis of Yeast Artificial Chromosome Clones
The technology of cloning large fragments of DNA in yeast as yeast artificial ch
