-
In Situ PCR Amplification of Intracellular mRNA
The polymerase chain reaction (PCR) is now commonly used in laboratories involve
-
Rapid (Ligase-Free) Subcloning of Polymerase Chain Reaction Products
The polymerase chain reaction (PCR) is a versatile, widely used method for the p
-
Polymerase Chain Reaction: Basic Principles and Routine Practice
The polymerase chain reaction (PCR) is a primer-mediated enzymatic amplification
-
Toward Effective PCR-Based Amplification of DNA on Microfabricated Chips
The polymerase chain reaction (PCR) has rapidly become the most valuable tool in
-
HPLC of DNA and PCR Products
The polymerase chain reaction (PCR) has rapidly become a standard laboratory tec
-
Production of Hybridization Probes by the PCR Utilizing Digoxigenin-Modified Nucleotides
The polymerase chain reaction (PCR) (1) is an efficient method for copying a fra
-
Dynamic Monitoring of Cellular Remodeling Induced by the Transforming Growth Factor-1
The plasticity of differentiated adult cells could have a great therapeutic pote
-
Purification of PDE6 Isozymes From Mammalian Retina
The photoreceptor phosphodiesterase (PDE6) is the central effector of visual tra
-
Inositol 1,4,5-Trisphosphate Mass Assay
The phosphoinositide cascade plays a central role in the transduction of many ex
-
Phosphatidylinositol(3,4,5)Trisphosphate (Ptdins(3,4,5)P3) Mass Measurement Using a Radioligand Disp
The phosphatidylinositol 3-kinases (PI 3-kinases), which play a pivotal role in
-
Expression and Purification of Recombinant Proteins Using the pET System
The pET System is the most powerful system yet developed for the cloning and exp
-
In Vivo Measurement of Tissue Oxygen Using Electron Paramagnetic Resonance Spectroscopy with Oxygen-
The partial pressure of oxygen (pO2 ) plays a determining role in the energy met
-
Study of MAPK Signaling Using Knockout Mice
The p42 and p44 mitogen-activated protein kinases (MAPKs) (p42/p44 MAPK, Erk2 /E
-
Post-electrophoretic identification of oxidized proteins
The oxidative modification of proteins has been shown to play a major role in a
-
Immunohistochemical Detection of Protein Oxidation
The oxidative modification of proteins by reactive oxygen species (ROS) and othe
