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Assessment of the Expression of Prostaglandin Synthase-2 in Swiss 3T3 Fibroblasts
Prostaglandin synthases are key enzymes in the prostanoid biosynthetic pathway.
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Using N,N,N,N-tetramethyl-p-phenylenediamine (TMPD) to Assay Cyclooxygenase Activity In Vitro
Prostaglandin endoperoxide synthase (PGH synthase), also known as cyclooxygenase
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A Proteomic Protocol to Identify Physiological Substrates of Pro-protein Convertases
Proprotein convertases (PCs) convert pro-proteins into their bioactive forms thr
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The 1,5-Disubstituted Tetrazole Ring as a cis-Amide Bond Surrogate
Proline occupies a special role among those amino acids incorporated into peptid
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Detection of Apoptosis Using Cyclic Luciferase in Living Mammals
Programmed cell death, apoptosis, is a crucial process involved in pathogenesis
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Plasmid Preparation on Sephacryl S1000
Production of pure plasmid DNA is a prerequisite for most laboratories engaged i
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RNA-based Studies Processing of Microdissected Tissue for Molecular Analysis
Processing of Microdissected Tissue for Molecular AnalysisRNA-based StudiesThis
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Crude Extract Immunodepletion
Problems:The main problem with immunodepletion of crude CSF extracts is that the
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Hybridization of Digoxigenin-Labeled Probes to Southern Blots and Detection by Chemiluminescence
Probes prepared with either digoxigenin- or biotin-modified nucleotides can be h
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Labeling of Double-Stranded DNA Probes with Biotin
Probes labeled with biotin have been used for hybridizations to Southern blots a
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Screening cDNA Libraries by Hybridization with Double-Stranded DNA Probes and Oligonucleotides
Probably the most commonly used method to screen a cDNA library is hybridization
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Polyacrylamide Gel Electrophoresis of Fluorophore-Labeled Carbohydrates from Glycoproteins
Prior to 1980, most methods for analysis of glycoprotein carbohydrates utilized
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SPR Biosensor as a Tool for Screening Prion Protein Binders as Potential Antiprion Leads
Prion diseases, also called transmissible spongiform encephalopathies (TSEs), ar
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Primer Extension Analysis of mRNA
Primer extension is a relatively quick and convenient means by which gene transc
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Methods to Isolate, Culture, and Study Osteoblasts
Primary cells are often desirable over clonal cell lines, because they are more
