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Sanger双脱氧法测序原理
DNA的复制需要：DNA聚合酶，单链DNA模板，带有3'-OH末端的单链寡核苷酸引物，4种dNTP（dATP、dGTP、dTTP和dCTP）。聚合酶用模
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DNA测序原理和方法
DNA测序原理和方法DNA序列测定分手工测序和自动测序，手工测序包括Sanger双脱氧链终止法和Maxam-Gilbert化学降解法。自动化测序实际上已成为当今
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测序（3'端测序，5'端测序）
问：刚做克隆，遇见测序过程中 有3'端测序，5'端测序，到底是怎么回事？为什么有两个测序结果？我应该按那一个来看测序正确还是不正确？答：测序都是
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DNA测序的基本原理及准备样品时的注意事项
一、测序的基本原理：测序的基本原理是Sanger末端终止法，在酶的作用下将原本是一条长达数百碱基的片断扩增成数百条片断，彼此间相差一个碱基。用电泳分离这些片断后
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染色体制备
一、 人外周血染色体制备1. 实验原理 人的外周血淋巴细胞培养方法是1960年由Moorhead 提出来的。正常情况下，人外周血小淋巴细胞都处在G1期（或G0期
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荧光素酶检测
IntroductionLuciferase can be used as a reporter gene to measure the activity of
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DNA Sequencing by Capillary Electrophoresis
IntroductionFor over 25 years, Applied Biosystems has been a pioneer in the fiel
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Summary: Illumina's Solexa Sequencing Technology
Here I present a brief overview of Solexa's sequencing-by-synthesis chemistr
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SOLiD Sequencing
Applied Biosystems has just launched their instrument, which supports their vers
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Sequencing Protocol
ABI SOLiD sequencing is a form of DNA sequencing. DNA sequencing encompasses bio
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ABI Solid Sequencing
ABI SOLiD sequencing is a form of DNA sequencing. DNA sequencing encompasses bio
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454 Sequencing
454 Life Sciences is a biotechnology company based in Branford, Connecticut spec
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测序技术
在分子生物学研究中，DNA的序列分析是进一步研究和改造目的基因的基础。目前用于测序的技术主要有Sanger等（1977）发明的双脱氧链末端终止法和Maxam和
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Protocol for Whole Genome Sequencing using Solexa Technology
The Solexa sequencing platform comprises all hardware, software, procedures and
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solexa sequencing
technology: illumina sequencing technologyThis breakthrough platform is based on
