小鼠单链抗体表达库所设计的VH和VL引物
小鼠单链抗体表达库所设计的 VH 和 VL 引物 VH FR1引物VL FR1引物15’-GGCTCGAGCAGGTT(G)CAGCTGC(A)AGC AGT
小鼠单链抗体表达库所设计的 VH 和 VL 引物
| VH FR1引物 | VL FR1引物 | |
| 1 | 5’-GGCTCGAGCAGGTT(G)CAGCTGC(A)AGC AGTCT(A)GG | 5’-CCTCTAGAGACATCCAGATGAC(A)CCAGTCTCC |
| 2 | 5’-GCTCGAGCAGGTG(A)CAGCTGG(A)T(A) GC(G)AGTCT(A)GG | 5’-CCTCTAGAGACATT(C)G(T)TGCTGACC(T)CAA (G)TCTCC |
| 3 | 5’-GGCTCGAGCAGGTCCAACTGCAGC(G)AGC (T)CTGG | 5’CCTCTAGAG(A)ACATTGTG(A)ATGACA(C)CAG(A)TCTCC |
| 4 | 5’-GGCTCGAGGAGGTGA(C)AGCTGGTGGAA (G)TCT(C)GG | 5’-CCTCTAGAGACATC(T)CAGA(C)TGACT(C)CAGTC TCC |
| 5 | 5’-GGCTCGAGGAG(A)GTGAA(T)GC(G)TGG TGGAGTCTGG | 5’-CCTCTAGAGGAGACATTGTGATGACCCAGTC |
| 6 | 5’-GGCTCGAGGAG(A)GTCCAGCTT(G)CA GCAGTCAGG | 5’-CCTCTAGAGATATTGTGA(C)TAACC(T)CAGG(T)A (C)TG(C)A(C)AC(G)T(C)C |
| 7 | 5’-GGCTCGAGGAGGTCCAGCTGCAA(G)CAGT CC(A)GG(T)A(G)C(G)C | 5’-CCTCTAGAGATATCG(C)T(A)T(G)ATGACC(A)CA GT(A)CTC(A)C |
| 8 | 5’-GGCTCGAGGAGGTTCAGCTT(G)CAGCAGT CTGG(T)A(G)GC | 5’-CCTCTAGAGCTGTTGTGA(G)TGACC(T)CAAACTCC |
| 9 | 5’-GGCTCGAGGAG(A)GTG(T)AAG(A)CTT (G)C(G)TC(A)GAGTCTGG | 5’-CCTCTAGAGATGTTT(G)TGATGACCCAA(C)ACTCC |
| 10 | 5’-GCTCGAGGATGTGCAGCTG(T)G(C)T(A) GGAGTCT(A)GG | 5’-CCTCTAGAGATGTTTTGATGAA(C)CCAAAC(T)TCC |
| 11 | 5’-GGCTCGAGCAGTCAGTGAAGGAGTCCGA GGG | 5’-CCTCTAGAGAA(G)AATG(C)TGCTCACCCAGTCTCC |
| 12 | 5’-GGCTCGAGGACTACTACATAAACTGGGTGA AAC | 5’-CCTCTAGAG(C)AAATTGTTCTCACCCAGTCTCC |
| 13 | 5’-CCTCTAGACAGGCTGTTGTGACTCAGGAATC | |
| 14 | 5’-GGACTAGTT(G)G(A)A(G)G(C)GAGACT GTGAGAGTGGTG(T)C | 5’-CCGAATTCTTTG(T)ATTTCCAGCTTGGTG(C)CCT (C)C |
| 15 | 5’-GGACTAGTTGC(A)A(G)GAG(A)ACAGT GACCA(G)G(T)A(G)GTCC | 5’-CCGAATTCTTTGAT(G)CTCCAGCTTGGTCCCCC |
| 16 | 5’-GGACTAGTTGAGGAGACG(A)GTGACTG A(T)GGTTC | 5’-CCGAATTCTTTT(C)AT(G)T(C)TCCAA(G)CTTT (G)GTCCCCG |
| 17 | 5’-GGACTAGTT(A)GC(A)AGAG(A)ACA(G) GTG(A)AG(C)A(G)GTGGTG(A)C | 5’-CCGAATTCA(G)CCGACAGTCAGTTTGGTT(A)CCTC |
| 18 | 5’-GGACTAGTT(C)GAGGAGACGGTGACCG (A)T(G)GGTC(G)C | 5’-CCGAATTCA(T)C(A)C(G)GACAGTGACCTT GGTTCCAC |
| 19 | 5’-CCGAATTCAGCGCGCTTCAGTTCCAGTTTGG |
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