配制Tris-甘氨酸SDS聚丙烯酰胺凝胶电泳分离胶所用溶液
配制Tris-甘氨酸SDS聚丙烯酰胺凝胶电泳分离胶所用溶液 溶液成分不同体积(ml)凝胶液中各成分所需体积(ml)5101520253040506%水2.65
配制Tris-甘氨酸SDS聚丙烯酰胺凝胶电泳分离胶所用溶液
| 溶液成分 | 不同体积(ml)凝胶液中各成分所需体积(ml) | |||||||
| 5 | 10 | 15 | 20 | 25 | 30 | 40 | 50 | |
| 6% | ||||||||
| 水 | 2.6 | 5.3 | 7.9 | 10.6 | 13.2 | 15.9 | 21.2 | 26.5 |
| 30%丙烯酰胺溶液 | 1.0 | 2.0 | 3.0 | 4.0 | 5.0 | 6.0 | 8.0 | 10.0 |
| 1.5mol/L Tris-HCl，pH 8.8 | 1.3 | 2.5 | 3.8 | 5.0 | 6.3 | 7.5 | 10.0 | 12.5 |
| 10%（w/v）SDS | 0.03 | 0.10 | 0.15 | 0.20 | 0.25 | 0.30 | 0.40 | 0.50 |
| 10%过硫酸胺 | 0.05 | 0.10 | 0.15 | 0.20 | 0.25 | 0.30 | 0.40 | 0.50 |
| TEMED | 0.004 | 0.008 | 0.012 | 0.016 | 0.02 | 0.024 | 0.032 | 0.04 |
| 8% | ||||||||
| 水 | 2.3 | 4.6 | 6.9 | 9.3 | 11.5 | 13.9 | 18.5 | 23.2 |
| 30%丙烯酰胺溶液 | 1.3 | 2.7 | 4.0 | 5.3 | 6.7 | 8.0 | 10.7 | 13.3 |
| 1.5mol/L Tris-HCl，pH 8.8 | 1.3 | 2.5 | 3.8 | 5.0 | 6.3 | 7.5 | 10.0 | 12.5 |
| 10%（w/v）SDS | 0.03 | 0.10 | 0.15 | 0.20 | 0.25 | 0.30 | 0.40 | 0.50 |
| 10%过硫酸胺 | 0.05 | 0.10 | 0.15 | 0.20 | 0.25 | 0.30 | 0.40 | 0.50 |
| TEMED | 0.004 | 0.008 | 0.012 | 0.016 | 0.02 | 0.024 | 0.032 | 0.04 |
| 10% | ||||||||
| 水 | 1.9 | 4.0 | 5.9 | 7.9 | 9.9 | 11.9 | 15.9 | 19.8 |
| 30%丙烯酰胺溶液 | 1.7 | 3.3 | 5.0 | 6.7 | 8.3 | 10.0 | 13.3 | 16.7 |
| 1.5mol/L Tris-HCl，pH 8.8 | 1.3 | 2.5 | 3.8 | 5.0 | 6.3 | 7.5 | 10.0 | 12.5 |
| 10%（w/v）SDS | 0.03 | 0.10 | 0.15 | 0.20 | 0.25 | 0.30 | 0.40 | 0.50 |
| 10%过硫酸胺 | 0.05 | 0.10 | 0.15 | 0.20 | 0.25 | 0.30 | 0.40 | 0.50 |
| TEMED | 0.004 | 0.008 | 0.012 | 0.016 | 0.02 | 0.024 | 0.032 | 0.04 |
| 12% | ||||||||
| 水 | 1.6 | 3.3 | 4.9 | 6.6 | 8.2 | 9.9 | 13.2 | 16.5 |
| 30%丙烯酰胺溶液 | 2.0 | 4.0 | 6.0 | 8.0 | 10.0 | 12.0 | 16.0 | 20.0 |
| 1.5mol/L Tris-HCl，pH 8.8 | 1.3 | 2.5 | 3.8 | 5.0 | 6.3 | 7.5 | 10.0 | 12.5 |
| 10%（w/v）SDS | 0.03 | 0.10 | 0.15 | 0.20 | 0.25 | 0.30 | 0.40 | 0.50 |
| 10%过硫酸胺 | 0.05 | 0.10 | 0.15 | 0.20 | 0.25 | 0.30 | 0.40 | 0.50 |
| TEMED | 0.004 | 0.008 | 0.012 | 0.016 | 0.02 | 0.024 | 0.032 | 0.04 |
| 15% | ||||||||
| 水 | 1.1 | 2.3 | 3.4 | 4.6 | 5.7 | 6.9 | 9.2 | 11.5 |
| 30%丙烯酰胺溶液 | 2.5 | 5.0 | 7.5 | 10.0 | 12.5 | 15.0 | 20.0 | 25.0 |
| 1.5mol/L Tris-HCl，pH 8.8 | 1.3 | 2.5 | 3.8 | 5.0 | 6.3 |
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