各种荧光标记基团的激发和发射波数据参数
荧光标记基团的激发和发射波长是广大科研工作者最关心的内容.下面就我们大家常用的多肽标记的各种荧光基团数据参数提供给大家.荧光染料激发波长，nm发射波长，nmFI
荧光标记基团的激发和发射波长是广大科研工作者最关心的内容.
下面就我们大家常用的多肽标记的各种荧光基团数据参数提供给大家.
荧光染料激发波长，nm发射波长，nm
FITC494518
5-FAM494522
TAMRA560582
Rhodamine B555580
Cy3550570
Cy5649670
从发射波长相近的角度考虑,上述荧光基团在用AlexFluor替换时,可参照下表
Alexa Fluor dyeAlternatives common dyes
Alexa Fluor 350Coumarin (MCA, AMC)
Alexa Fluor 488Cy2 or Fluorescein (FITC, FAM)
Alexa Fluor 555Cy3 or Tetramethylrhodamine (TRMRA)
Alexa Fluor 568Rhodamine Red
Alexa Fluor 594Texas Red
Alexa Fluor 647Cy5
Alexa Fluor 680Cy5.5
Alexa Fluor 750Cy7
| 荧光分子 | 颜色 | 激发波长 | 发射波长 |
| Alexa Fluor 350 | Blue | 346 nm | 442 nm |
| Alexa Fluor 405 | Blue | 402 nm | 421 nm |
| Pacific Blue | Blue | 410 nm | 455 nm |
| DAPI | Blue | 340 nm | 488 nm |
| AMCA | Blue | 350 nm | 450 nm |
| Cascade Blue | Blue | 400 nm | 420 nm |
| EviTag(TM) quantum dots - Lake Placid Blue (490 nm) | Blue | <400 nm | 490 nm |
| Fluoroblue | Blue | 350 nm | 450 nm |
| Image-Blue | Blue | 380 nm | 465 nm |
| Marina Blue | Blue | 365 nm | 460 nm |
| Pacific Blue | Blue | 410 nm | 455 nm |
| Cy2 | Green | 491 nm | 509 nm |
| Alexa Fluor 430 | Green | 434 nm | 539 nm |
| Alexa Fluor 488 | Green | 495 nm | 519 nm |
| Alexa Fluor 488 FluoroNanogold | Green | 495 nm | 519 nm |
| Alexa Fluor 500 | Green | 503 nm | 525 nm |
| Alexa Fluor 514 | Green | 518 nm | 540 nm |
| FITC | Green | 490 nm | 520 nm |
| Fluorescein | Green | 494 nm | 518 nm |
| HNP | Green | 290 nm | 509 nm |
| 5-FAM | Green | 494 nm | 522 nm |
| 6-FAM | Green | 492 nm | 517 nm |
| BODIPY FL | Green | 505 nm | 513 nm |
| CMNB-caged Fluorescein | Green | 494 nm | 518 nm |
| Cyanine 2 | Green | 492 nm | 510 nm |
| DTAF | Green | 495 nm | 520 nm |
| DTAF/FITC | Green | 495 nm | 520 nm |
| dUTP-Biotin and Avidin FITC | Green | 495 nm | 520 nm |
| dUTP-FITC | Green | 495 nm | 520 nm |
| EviTag(TM) quantum dots - Adirondack Green (520 nm) | Green | <400 nm | 520 nm |
| EviTag(TM) quantum dots - Catskill Green (540 nm) | Green | <400 nm | 540 nm |
| FITC (Fluorescein) | Green | 495 nm | 520 nm |
| Fluorescein-5-EX, succinimidyl ester (FSE) | Green | 495 nm | 520 nm |
| Hi FITC | Green | 495 nm | 520 nm |
| JOE | Green | 520 nm | 548 nm |
| MFP488 | Green | 501 nm | 523 nm |
| Oregon Green 488 | Green | 496 nm | 524 nm |
| Oregon Green 514 | Green | 511 nm | 530 nm |
| Qdot 525 | Green | 488 nm | 515 nm |
| TET | Green | 521 nm | 536 nm |
| CAL Fluor(R) Gold 540 | Green | 522 nm | 544 nm |
| NL493 | Green | 493 nm | 514 nm |
| GFP | Green | 395/475 nm | 509 nm |
| Cy3 | Yellow | 548 nm | 562 nm |
| Cy3B | Yellow | 558 nm | 572 nm |
| Alexa Fluor 532 | Yellow | 531 nm | 554 nm |
| Alexa Fluor 546 | Yellow | 556 nm | 573 nm |
| Alexa Fluor 555 | Yellow | 555 nm | 565 nm |
| R-Phycoerythrin | Yellow | 480, 546, 565 nm | 578 nm |
| B-Phycoerythrin | Yellow | 546, 565 nm | 575 nm |
| HNP/Fast Red TR | Yellow | 290 nm | 562 nm |
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